Easychem TOX Early Warning

The result of intensive research activity, EASYCHEM TOX Early Warning is an exclusive direct-reading analyser using robotic technology, which allows fully automatic measurements of acute toxicity using rehydrated cultures of luminous bacteria.

EASYCHEM TOX Early Warning is a discrete analyser equipped with a colour touchscreen monitor housed in an industrial cabinet, comprising a refrigerated compartment for bacteria, reagents, calibrators and control solutions. A rotating mechanical arm performs aspiration, transfer and dispensing of solutions into 80 cuvettes located in a rotating, thermostatted reaction plate, including a luminometric reading system and integrated with an automatic cuvette washing station.

Measurement of acute toxicity using luminescent bacteria

To allow extended use of rehydrated bacterial cultures for up to 10 days, proprietary marine bacteria have been selected, grown, stabilised, and freeze-dried in ready-to-use vials, in accordance with ISO 11348-3. This method ensures that, after automatic rehydration, the microorganisms retain a measurable bioluminescence signal and unchanged sensitivity to various reference substances, such as zinc sulphate and mercury chloride, in accordance with the aforementioned ISO or GB-T standard. The system is programmed to manage numerous bacterial vials sequentially in a fully automated manner, enabling a high frequency of automatic analysis over an extended period (approximately one month of autonomy for unattended operation).

EASYCHEM TOX Early Warning - Robotic Technology Direct Reading Analyser

Unattended acute toxicity monitoring

The timing and frequency of analysis, limits, and control parameters are user-programmable and automatically managed. Continuous measurement of blanks and samples at a predetermined frequency of 40 seconds allows for near real-time monitoring of the inhibition kinetics, providing information on the residual luminescence of the sample after just a couple of minutes from the start of the analysis, and therefore on the sample's contamination level. Following a toxicity alarm, the system automatically reacts by performing a sample replica and positive controls with onboard known solutions, in order to confirm the detected toxicity, significantly reducing the risk of detecting false positives.

Standard data interfaces

The Easychem TOX Early Warning multiparameter analyser, equipped with a colour graphic touchscreen display, provides data locally via RS-232 serial output with separate 4-20 mA analogue outputs, and remotely via a web server for data viewing and presentation, via FTP, email, and by sending data and/or alarms via SMS to authorised operators' mobile phones.

Advantages and benefits

Fully automated analysis of enhanced toxicity, in accordance with standard ISO 11348-3;
Long operating life; low maintenance, low operating cost;
Very low reagent consumption; no preparation time; low cost of consumables;
Simple operation; “plug & play” analysis, no particular specialisation is required of the operator.

Applications

Parameters analysed

SKU PWR-MICC-6 Category Online Analysers Tag Acute toxicity

Technical Specifications

Technology	Online real-time toxicity trend - DAMTA* Analytical monitoring and toxicity assessment device
DETECTOR AND READING	High-sensitivity light meter - Direct reading
Microspecimen reaction cuvettes	80, reusable after washing and quality control; 6.0 mm
REACTION PLATE TEMPERATURE	Adjustable from 15 to 25 °C, accuracy: ±0.1 °C
LEVEL SENSORS	Samples/Reagents/Waste
SAMPLE REQUIREMENTS	100-200 m³/h at ambient pressure
SAMPLE/AMBIENT TEMPERATURE	Sample: 5 to 35 °C; Ambient: 5 to 28 °C; Relative humidity at 28 °C <40%
Feeding	110/230 V AC, 50/60 Hz
Energy consumption	400 W
DIMENSIONS/WEIGHT	160 (W) x 80 (D) x 50 (H) cm / 100 kg without reagents
PC and Software	PC panel with integrated touchscreen, dedicated software
Ethernet port Data transmission via FTP GPRS DATA TRANSMISSION ANALOGUE OUTPUT DIGITAL OUTPUT - ALARM	Standard Standard via Ethernet port or with optional 3G modem With optional 3G modem 4-20 mA - optional Optional
REAGENTS	Lyophilised bacterial vials: rehydration and direct use Analysis swabs and positive controls: ready for use
Refrigerated reagent tray	Programmable positive reference check
Calibration Interval	Reference control - programmable
REPLACEMENT OF REAGENTS	30 days with maximum frequency; with lower frequencies, reagent replacement up to 3 months
SAMPLING	Continuous data in the sampling container, 2-minute moving average
Measurement frequency	Programmable; maximum frequency: 2.5 minutes
EVENT RESPONSE TIME	Minimum 60 seconds - Maximum 150 seconds
Analysis time until final result	From 1 to 15/30 minutes, depending on sample toxicity
Real-time calculation	Real-time calculation of all parameters required by standards UNI EN ISO 11348-3 and GB-T 15441
EMPTY FIELDS (Checking for false negatives and false positives)	Programmable for one vacuum per sample
SAMPLE REPLICA AND POSITIVE CONTROL (Validation of false positives)	Programmable after any report of toxicity The analyser performs a new test on the sample with a maximum of 5 dilutions, one positive control and calculates the EC50 in case of toxicity above a predefined threshold.